Table 3.
Primer sequences for target and reference genes used for RT-qPCR.
| Symbol | Forward primer (5’ to 3’) | Reverse primer (5′ to 3′) | Amplicon size (bp) | RT-qPCR Efficiency (%) | |
|---|---|---|---|---|---|
| Reference genes | ACTB | GCTACAGCTTCACCACCACA | TCTCCTGCTCGAAATCCAGT | 90 | 106.8 |
| GAPDH | GCAGATGCAGGTGCTGAGTA | GACACCCATCACAAACATGG | 144 | 102.2 | |
| HMBS | GACTGACAGCGTGGTTGAGA | CCAGCTCTTTGGTGAAGAGG | 143 | 102.7 | |
| HPRT1 | AAGTGGCCAGTTTGTTGGTC | GTAGTCGAGGGCGTATCCAA | 110 | 95.8 | |
| SDHA | TTCCCGTTTTGCCTACGGTG | CTGCCTCGCCACAAGCATAT | 126 | 101.8 | |
| TUBB1 | CCGCATCAGCGTCTACTACA | GTCTGAAGATCTGCCCGAAG | 125 | 102.9 | |
| YWHAZ | TTCTTGATCCCCAATGCTTC | TTGTCATCTCCAGCAGCAAC | 104 | 97.1 | |
| Target genes | CASP8 | GGTGAGCAGCAAGATTGACA | CTGCCTCTGCTCCCATTTAG | 110 | 102.2 |
| IL22 | CAGGAATCGCACCTACACCT | CGGTTGTTCTCCCTGATGTT | 108 | 107.2 | |
| TLR3 | CCCTGATGGAGTGTTTGCTT | CCAGGGTTTTGAAAGGATCA | 95 | 116.9 |