Fig. 5.

TRIM7 exerted its antitumor effect through modulation of the Src-mTORC1-S6K1 axis. a–d HepG2 and Huh7 cells were co-transfected with Flag-TRIM7 plasmid and HA-Src plasmid, and successful overexpression of TRIM7 and Src in the transfected cells was detected by western blot (a). The proliferation (b), invasion (c), and colony formation (d) of the transfected HCC cells were further detected and analyzed. e–h HepG2 cells were transfected with Si-NC, Si-TRIM7-1, or Si-TRIM7-2 before treatment with rapamycin (100 nM) for 48 h, and the protein levels of p-S6K1, p-S6, Src, and p-Src in the transfected cells were detected by western blot (e). The proliferation (f), invasion (g), and colony formation (h) of the transfected HepG2 cells were further detected and analyzed. *P < 0.05, **P < 0.01, and ***P < 0.001 for statistical analysis of the indicated groups. Scale bar in c, g: 100 μm. The presented figures are representative of data from at least three independent experiments