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. 2019 Dec 20;27(6):1998–2013. doi: 10.1038/s41418-019-0483-6

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© The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a HeLa cells stably expressing control (shV) or hnRNP F shRNA (#1) were passaged over time and the average telomere length was examined at different population doublings (PD) by a telomere restriction fragment assay. b Data from a were quantified and graphed. Error bars represent means ± SD (n = 3). Statistical analysis was performed using Student's t test (**P < 0.01, ***P < 0.001). c HeLa cells stably expressing control (shV) or hnRNP H1 shRNA (#1) were passaged over time and the average telomere length was examined at different PD by a telomere restriction fragment assay. d Data from c were quantified and graphed. Error bars represent means ± SD (n = 3). Statistical analysis was performed using Student's t test (**P < 0.01, ***P < 0.001). e HeLa cells stably expressing control (shV) or hnRNP F shRNAs (#1 and #2) transfected with FLAG-hTERT plasmid for 48 h and then cells were stained for FLAG-hTERT using anti-FLAG antibody (red), Coilin (a specific Cajal body marker, green), and nuclei (DAPI, blue). Representative immunofluorescence images are shown. White arrow heads indicate the overlapped signals. Scale bar, 10 μm. The experiments were repeated three times.