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. 2019 Dec 20;27(6):1998–2013. doi: 10.1038/s41418-019-0483-6

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© The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a hMSCs cells stably expressing pLKO.1-vector or pLKO.1-hnRNP F (#1 or #2) were generated and hnRNP F knockdown was confirmed by WB analysis. Then the stable hMSCs cell lines were used to perform following assays: b TRAP assay, data were quantified and graphed. Error bars represent means ± SD (n = 3). Statistical analysis was performed using Student's t test (***P < 0.001); c CCK-8 cell proliferation assay, error bars represent means ± SD (n = 3). Statistical analysis was performed using Student's t test (***P < 0.001); d Cells were stained for Ki67 using anti-Ki67 antibody (green), and nuclei (DAPI, blue). Representative immunofluorescent images are shown. Scale bar, 100 μm. The experiments were repeated three times. Data were quantified and graphed. Error bars represent means ± SD (n = 3). Statistical analysis was performed using Student's t test (*** P < 0.001); e Cell cycle analysis, error bars represent means ± SD (n = 3). Statistical analysis was performed using Student's t test (***P < 0.001). f hMSCs cells passaged over time and cell lysates of different PDs were subjected to the WB analysis for the indicated proteins. g hMSCs cells described in a were assessed by SA-β-gal activity assay. Scale bar, 10 μm. Data were quantified and graphed. Error bars represent means ± SD (n = 3). Statistical analysis was performed using Student's t test (*** P < 0.001). h hMSCs cells stably expressing pHBLV-vector or pHBLV-hnRNP F or H1 were subjected to the SA-β-gal activity assay. Scale bar, 10 μm. Data were quantified and graphed. Error bars represent means ± SD (n = 3). Statistical analysis was performed using Student's t test (***P < 0.001).