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. 2019 Dec 19;27(6):1938–1951. doi: 10.1038/s41418-019-0473-8

Fig. 3. USP33 knockout promotes the apoptosis of prostate cancer cells.

Fig. 3

a Cell viability was measured by CCK8 assay in PC3 cells after depleting USP33 and transfecting Mock or WT-USP33 vector for 48 h as indicated. b BrdU incorporation assay was performed in the cells transfected as in a at 24 h. c USP33+/+ and USP33−/− PC3 cells were transfected with Mock, WT-USP33 or Mut-USP33 vector as indicated for 48 h and then treated with DMSO or docetaxel (Doc) (10 nM) for 48 h as indicated. Annexin V+ apoptotic cells were quantified by Annexin V/PI assay. Data and error bars in a to c represent the mean ± s.d. of triplicate samples derived from one representative experiment. Similar results were obtained in three independent experiments. ***P < 0.001; ns, not significant (one-way ANOVA followed by Bonferroni multiple comparison using PRISM software). d USP33+/+ and USP33−/− PC3 cells were transfected as in c and then treated with DMSO or docetaxel (Doc) (10 nM) for 24 h as indicated. The indicated molecules were examined by western blot assay. One representative experiment of three is shown. Similar results were obtained in three independent experiments.