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. 2019 Dec 19;27(6):1938–1951. doi: 10.1038/s41418-019-0473-8

Fig. 7. DUSP1 and USP33 synergistically inhibit docetaxel-induced activation of JNK and apoptosis of PC3 cells.

Fig. 7

a PC3 cells were cotransfected with control (Ctrl) or USP33 siRNA (USP33 RNAi #1) and Mock or Myc-DUSP1 vector for 48 h and then treated with DMSO or docetaxel (10 nM) for 48 h as indicated. Annexin V+ apoptotic cells were quantified by Annexin V/PI assay. b PC3 cells were cotransfected with control (Ctrl) or USP33 siRNA (USP33 RNAi #1) and Mock or Myc-DUSP1 vector for 48 h as indicated in the presence of docetaxel (10 nM) for 24 h, and the indicated molecules were examined by western blot analysis. One representative experiment of three is shown. Similar results were obtained in three independent experiments. c USP33+/+ and USP33−/− PC3 cells were transfected with control (Ctrl) or DUSP1 siRNAs for 48 h as indicated and then treated with docetaxel (10 nM) for 48 h. Annexin V+ apoptotic cells were quantified by Annexin V/PI assay. Data and error bars in a and c represent the mean ± s.d. of triplicate samples derived from one representative experiment. Similar results were obtained in three independent experiments. *P < 0.05; ***P < 0.001; ns, not significant (one-way ANOVA followed by Bonferroni multiple comparison using PRISM software). d USP33+/+ and USP33−/− PC3 cells were transfected with control (Ctrl) or DUSP1 siRNA for 48 h as indicated and then treated with docetaxel (10 nM) for 24 h, and the indicated molecules were examined by western blot analysis. One representative experiment of three is shown. Similar results were obtained in three independent experiments.