Fig. 9.

Spreading of αSYN pathology after inoculation of different PMCA-amplified αSYN strains. a, b Representative photomicrographs of PαSYN pathology in four different brain regions 5 months after inoculation of PMCA-amplified αSYN assemblies without (a) or with (b) rAAV2/7-αSYN expression. The selected brain regions are the following: (1) and (2) adjacent sections of caudate putamen (CPu); (3) SNpc and cerebral peduncle (cp); (4) lateral part of the SNpc and SNpr. Black arrows represent PαSYN positive cells and open arrows show PαSYN-positive outgrowths. Scale bars represents 200 µm (left) and 50 µm (right). (c–d) Heat map for the spreading of αSYN pathology towards different brain regions after inoculation of PMCA-amplified αSYN assemblies without (c) or with (d) rAAV2/7-αSYN expression. The heat map ranges from low (green) tot very high (red). A total of three animals per group was included. The data were presented as heat maps to semi-quantitatively illustrate the distribution of αSYN pathology throughout the brain. Each panel represents a coronal plane (bregma 2.16, 1.20, − 1.80, − 4.36, − 5.28, − 5.76, − 6.24 mm) for each group. The left column presents sagittal views of the corresponding coronal planes