Fig. 7.

HUVECs treated with ADMA resulted in reduced NO production with no significant change in eNOS protein levels. Confluent HUVECs were treated with ADMA (25, 50, and 100 μM) for 24 h. a NO production was represented by nitrite and nitrate using Griess colorimetric assay and normalized to protein concentration (n = 3 per group). b Representative images and densitometry analysis of eNOS protein were detected by western blot, and the independent experiment was repeated for three times. GAPDH was used as internal references. Values are expressed as mean ± SD. *P < 0.05 compared with “control”