Fig. 3.

Effect of in vivo treatment of metformin on ovulation pattern. Swiss albino mice were treated with 50, 100, and 200 mg/kg body weight of metformin daily for 4 weeks. After the completion of the treatment, mice were superovulated with PMSG (5 IU) and hCG (10 IU). The OCCs were collected from the oviduct in KSOM media and denuded by incubating OCCs in KSOM media supplemented with hyaluronidase (0.1 mg/mL). MII oocytes were collected and the data was expressed as mean ± SE of MII oocytes retrieved per animal (n = 8)