Table 4.
The effect of in vitro exposure of various concentrations of metformin on developmental competence of 2-cell-stage embryos cultured in KSOM medium (24 embryos per group)
| Metformin concentration (μg/mL) | 2.5 dpc | 3.5 dpc | 4.5 dpc | ||||
|---|---|---|---|---|---|---|---|
| 6–8 cell rate (%) | Morula rate (%) | Blastocyst rate (%) | Blastocyst rate (%) | Hatching rate (%) | Total cell number | TUNEL index | |
| 0 | 95.83 (23) | 95.83 (23) | 70.83 (17) | 91.67 (22) | 29.17 (7) | 83.04 ± 3.78 | 5.72 ± 0.77 |
| 10 | 100 (24) | 100 (24) | 79.17 (19) | 95.83 (23) | 33.33 (8) | 90.09 ± 3.65 | 4.38 ± 0.76 |
| 25 | 95.83 (23) | 95.83 (23) | 50.00 (12) | 83.33 (20) | 12.50 (3) | 88.30 ± 4.18 | 7.37 ± 1.27 |
| 50 | 100 (24) | 100 (24) | 54.17 (13) | 95.83 (23) | 25.00 (6) | 71.82 ± 4.33 | 5.36 ± 1.63 |
| 100 | 100 (24) | 100 (24) | 41.67 (10) | 95.83 (23) | 8.33 (2)a | 53.61 ± 2.90a | 2.05 ± 0.89 |
| 250 | 100 (24) | 100 (24) | 7.69 (2)c | 7.69 (2)c | 0b | - | - |
| 500 | 37.50 (9)a | 25 (6)c | 0c | 0c | 0b | - | - |
The developmental potential data were expressed as percentage (%). The total cell number and TUNEL index were expressed as mean ± SE
aP < 0.01, bP < 0.001, cP < 0.0001 compared with the control group