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. 2020 May 22;10:8587. doi: 10.1038/s41598-020-65468-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sonopores developed in the plasma membrane of cells after treatment with ultrasound in the presence and absence of MB (A) untreated MDA MB 231 cells (B) ultrasound treatment in absence of MB, dotted circles show pores. The black circled pore is shown in the inset (C) ultrasound treatment in the presence of MB, a magnified view of the dotted area is shown in the image (D). (D) Dotted circles surround pores and black circled pore is shown in the inset. B16F10 melanoma cells (E) untreated (F) treated with only ultrasound, (G) treated with ultrasound in presence of MB; the dotted area is magnified in image H, (H) dotted circles surround pores and black dotted circle pore is shown in the inset.