Fig. 3. The CGATA site at c-Kit intron 2 is a regulatory site bound by GATA-1 in MEL cells.

a Schematic of GATA sites in the c-Kit gene locus²¹. b Sequencing confirms that a (CG > TT)ATA mutation was introduced at c-Kit intron 2. c The mRNA expression level of c-Kit in wild type and (CG > TT)ATA mutant clones, n = 11 biologically independent cells for WT, n = 4 biologically independent cells for mutant, mean ± standard deviation. Measurements were taken from distinct samples (separate clonal cell populations). d GATA-1 ChIP qPCR in wild type and mutant (CG > TT)ATA clones, n = 4 biologically independent cells, mean ± standard deviation. The promoter region of Klf1 served as a positive control (+ve). An upstream region distal to the c-Kit gene was used as a negative control (−ve). All GATA consensus binding sites with evidence of GATA-1 binding in ChIP-seq studies in the c-Kit gene locus have been tested (+5, +7, +33, +49.5, +58 kb; see a). All experiments were performed in undifferentiated MEL cells. Source data are provided as a Source data file.