Table 2.
Protein composition of OMVBp134 and OMV Bp IP91determined by LC–MS
| Protein No.a | Gene | %Cov | Name | MW | PI | Length(aa) | Subcellular localisation |
|---|---|---|---|---|---|---|---|
| 1 | BP2759 | 2.743 | Putative thiolase | 41.473 | 6.8 | 401 | periplasm |
| 2 | BP2377 | 1.243 | Putative AMP-binding protein | 62.3 | 5.8 | 563 | cytoplasm |
| 3 | BP2818 | 26.04 | Lipoprotein | 28.7 | 7.7 | 265 | cytoplasm |
| 4 | BP0182 | 6.135 | Iron-sulfur cluster carrier protein | 38.2 | 6.2 | 163 | cytoplasm |
| 5 | BP3266 | 4.898 | Ribulose-phosphate 3-epimerase | 26.426 | 5.8 | 245 | cytoplasm |
| 6 | BP0965 | 28.64 | Antioxidant protein | 23.777 | 5.6 | 213 | cytoplasm |
| 7 | BP0205 | 34.04 | Putative exported protein | 11.77 | 8.4 | 114 | Extracellular |
| 8 | ahpC | 23.08 | Alkyl hydroperoxide reductase | 18.38 | 5.1 | 172 | cytoplasm |
| 9 | glcD | 3.206 | Glycolate oxidase subunit | 53.2 | 5.29 | 499 | cytoplasm |
| 10 | sbp | 60.47 | Sulfate-binding protein | 37.8 | 7.79 | 344 | Outer membrane |
| 11 | smoM | 21.7 | Putative periplasmic solute-binding protein | 40.01 | 7.36 | 364 | cytoplasm |
| 12 | BP0558 | 14.79 | Amino acid-binding periplasmic protein | 36.1 | 6.6 | 338 | periplasm |
| 13 | BP3809 | 20.31 | Uncharacterized protein | 34.1 | 6.23 | 320 | cytoplasm |
| 14 | BP2891 | 13.79 | Uncharacterized protein | 22.641 | 6 | 203 | cytoplasm |
| 15 | BP3465 | 4.167 | Uncharacterized protein | 18.809 | 6.2 | 168 | cytoplasm |
| 16 | BP2782 | 32.88 | Lipoprotein | 8.056 | 4.7 | 73 | Outer membrane |
| 17 | adhI | 9.239 | S-(hydroxymethyl)glutathione dehydrogenase | 39.4 | 6.2 | 368 | periplasm |
| 18 | minD | 60.89 | Site-determining protein | 29.6 | 5.2 | 271 | cytoplasm |
| 19 | BP0103 | 59.2 | Probable class IV aminotransferase | 32.2 | 5.4 | 299 | cytoplasm |
| 20 | sdhB | 31.93 | Succinate dehydrogenase iron-sulfur subunit | 27.2 | 6 | 238 | cytoplasm |
| 21 | BP3757 | 17.27 | Putative ABC transporter, ATPbinding protein | 29.6 | 5.2 | 278 | cytoplasm |
| 22 | BP1300 | 44.21 | Putative glutathione transferase | 26.4 | 6.2 | 233 | cytoplasm |
| 23 | BP0961 | 9.639 | Electron transfer flavoprotein beta subunit | 26.819 | 5.7 | 249 | cytoplasm, periplasm |
| 340 | |||||||
| 24 | BP2963 | 5.294 | Putative membrane protein | 40.055 | 7.6 | Outer membrane | |
| 25 | BP1702 | 3.409 | Probable enoyl-CoA hydratase/isomerase | 28.556 | 5.7 | 261 | cytoplasm |
| 26 | odhB | 1.98 | oxoglutarate dehydrogenase complex | 41.825 | 5.3 | 404 | cytoplasm |
| 27 | BP1616 | 27.71 | Putative DNA-binding protein | 18.52 | 6.2 | 166 | cytoplasm |
| 28 | BP3550 | 2.545 | AraC-family regulatory protein | 30.6 | 9 | 275 | cytoplasm |
| 29 | bopN | 1.918 | Putative outer protein N | 38.99 | 5 | 365 | cytoplasm |
| 30 | BP2533 | 46.07 | Inorganic pyrophosphatase | 20.042 | 5.3 | 178 | cytoplasm |
| 31 | BP0361 | 25.79 | Uncharacterized protein | 16.88 | 5.2 | 159 | Outer membrane |
| 32 | gltA | 13.99 | Citrate synthase | 48.46 | 6.03 | 436 | cytoplasm |
| 33 | sdhA | 4.899 | Succinate dehydrogenase flavoprotein subunit | 64.8 | 6.1 | 596 | cytoplasm |
| 34 | lpdA | 5.872 | Dihydrolipoyl dehydrogenase | 62.3 | 6.1 | 596 | cytoplasm |
| 35 | BP3277 | 45.45 | Putative enoyl-CoA hydratase | 30.1 | 6.2 | 275 | cytoplasm |
| 36 | mmsB | 19.19 | 3-hydroxyisobutyrate dehydrogenase | 29.6 | 5.7 | 297 | cytoplasm |
| 37 | BP2434 | 2.828 | Periplasmic serine endoprotease DegPlike | 52.1 | 7.6 | 495 | periplasm, Outer membrane |
Identifications were confirmed by LC- MS/MS ion search and significant matches (p < 0.05) were retained (Swiss-Prot protein database).
a:
Protein numbering refers to spot numbers in Fig. 4.