Table 1.

Neutralization validation results of hydrogen peroxide 7% solution

Neutralizer	Sodium thiosulfate in letheen broth						MnO2 in letheen broth			MnO2 in D.Wb			FeCl3 in D.W
concentration (w/v)	0.5	0.6	1	1.5	2	3	0.3	0.5	1	0.3	0.5	1	0.5	1
Recovery- test group (CFU/ml)	<10a	<10	<10	<10	<10	<10	97	99	95	98	98	97	<10	<10
Recovery- peptone group (CFU/ml)	100	93	91	86	87	78	98	100	94	99	99	96	96	94
Recovery- viability group (CFU/ml)	101	95	96	98	99	98	99	101	95	100	99	97	99	98
Efficacy (%)b	<10	<10	<10	<10	<10	<10	99	98	100	99	99	100	<10	<10
Toxicity (%)c	99	98	95	88	88	80	99	99	99	99	100	99	97	96

^aThe results are mean of triplicate counts;

^bRatio of test tube to viability tube count;

^Cratio of peptone control group to viability group.

^bD.W is stand for distilled water