Table 2.
The results of carrier test for 7% H2O2 according to AOAC MB 15-03 standard. Each carrier was loaded with 5×105 CFU of Bacillus subtilis spores.
Contact time (min) | Primary tube (neutralizer) | Secondary tube (medium) | Result | Positive control | Control media | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|
(1) | (2) | (3) | (4) | (5) | (1) | (2) | (3) | ||||
2 | +a | + | + | + | + | + | + | + | 0b | 0 | 0 |
5 | + | + | + | + | + | + | + | + | 0 | 0 | 0 |
8 | + | + | + | + | + | + | + | + | 0 | 0 | 0 |
10 | + | + | + | + | + | + | + | + | 0 | 0 | 0 |
13 | + | + | + | + | + | + | + | + | 0 | 0 | 0 |
15 | + | + | + | + | + | + | + | + | 0 | 0 | 0 |
17 | + | + | + | + | + | + | + | + | 0 | 0 | 0 |
20 | + | + | + | + | + | + | + | + | 0 | 0 | 0 |
25 | 0 | + | + | + | + | + | + | + | 0 | 0 | 0 |
30 | 0 | + | + | + | + | + | + | + | 0 | 0 | 0 |
60 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | + | 0 | 0 | 0 |
growth,
no growth