Table 1.
Comparison of growth kinetics between DP-MSCs and BMSCs.
Cell growth assay | ||||||
---|---|---|---|---|---|---|
Observation | DP-MSC |
BMSC |
Repeated measure ANOVA—general linear model (GLM) |
|||
Mean | ±SD | Mean | ±SD | Source | F | |
Day 1 | 10,666.67 | 500.00 | 10,000.00 | 0.00 | Corrected model | 838.48** |
Day 2 | 14,500.00 | 750.00 | 11,333.33 | 1000.00 | Intercept | 39,800** |
Day 3 | 24,000.00 | 866.03 | 17,333.33 | 4000.00 | Group | 640.12** |
Day 4 | 32,000.00 | 866.03 | 23,333.33 | 2645.75 | Days | 1674** |
Day 5 | 44,333.33 | 1000.00 | 33,333.33 | 4000.00 | Group × days | 27.34** |
Day 6 | 68,166.67 | 1750.00 | 55,000.00 | 1887.46 | ||
Day 7 | 80,000.00 | 3968.63 | 60,000.00 | 3774.92 | ||
Day 8 | 82,166.67 | 3682.73 | 63,333.33 | 3307.19 | ||
Day 9 | 82,500.00 | 4520.79 | 66,000.00 | 3464.10 |
Population doubling time and clonogenic assay | |||||
---|---|---|---|---|---|
Parameter | DP-MSCs |
BMSCs |
t value | ||
Mean | ±SD | Mean | ±SD | ||
Doubling time (h) | 31.11 | 2.22 | 51.83 | 0.45 | 18.680** |
Clonogenic assay (no of colonies) | 50.00 | 2.01 | 24.00 | 1.00 | 26.00** |
**P < 0.001.
ANOVA, analysis of variance; DP-MSCs, dental pulp mesenchymal stem cells; SD, standard deviation.