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. 2020 May 13;2020:3145182. doi: 10.1155/2020/3145182

Figure 2.

Figure 2

Apatinib inhibited OC cell migration. (a, b) The migration of A2780, SKOV-3, and CAOV-3 cells after treatment with apatinib (0, 10, and 20 μM) for 24 h was assessed using the transwell assay. The invaded cells on the bottom surface of the filters were stained, and the cell numbers were counted by ImageJ software. (c, d) The movement ability of three OC cells after treatment with apatinib (0, 10, and 20 μM) for 24 h was detected using wound healing assays. The gap distance that was calculated by ImageJ software was used to measure the movement ability. (e) After treatment with 20 μM apatinib for 24 h, protein levels of the EMT markers E-cadherin, N-cadherin, vimentin, and metastasis-associated protein, MMP9, in three OC cells were determined by western blotting. (f) The relative western blot gray values are shown in the histogram. Data are presented as the mean ± SD of three independent experiments. APA: apatinib.∗P < 0.05, ∗∗P < 0.01, compared with the control groups.