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. 2020 May 13;2020:3145182. doi: 10.1155/2020/3145182

Figure 6.

Figure 6

VEGFR2 regulates the Nrf2 pathway, and apatinib remains effective at a low level of VEGFR2 in OC. (a) Nrf2 had a significant positive relationship with VEGFR2 based on the GEPIA database. (b, c) The protein and mRNA levels of VEGFR2 were examined in three ovarian cancer cell lines by western blotting and qRT-PCR, respectively. A2780 cells were transfected with VEGFR2 or empty plasmid. (d) The protein levels of VEGFR2 and Nrf2 were tested by western blotting and (e) the increased mRNA levels of VEGFR2, Nrf2, HO-1, GCLC, and GCLM were determined by qRT-PCR. SKOV-3 cells were transfected with VEGFR2 or control siRNA. (f) The protein levels of VEGFR2 and Nrf2 were tested by western blotting and (g) the decreased mRNA levels of VEGFR2, Nrf2, HO-1, GCLC, and GCLM were tested by qRT-PCR. (h) The mRNA levels of VEGFR2 significantly decreased in OC compared with normal ovarian surface epithelium based on the dataset of Oncomine. (i) VEGFR2 mRNA expression was decreased in OC compared with normal tissues based on GEPIA database. (j) Representative immunohistochemical staining images of VEGFR2 in different pathological subtypes of OC and normal tissues based on the HPA database. Scale bar = 50 μm. SKOV-3 cells were transfected with VEGFR2 or control siRNA. (k) The protein levels of VEGFR2 were reduced after incubation with 20 μM apatinib. (l) The cell viability and (m) the migration of transfected SKOV-3 cells were suppressed by apatinib. Scale bar = 100 μm. The relative western blot gray values are shown in the histogram. Data are presented as the mean ± SD of three independent experiments. APA: apatinib; TPM: transcripts per million.". ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, compared with the control groups.