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. 2020 May 5;117(20):11136–11146. doi: 10.1073/pnas.1917174117

Fig. 2.

Fig. 2.

Atg7-deficient Lgr5+ISC apoptosis is dependent on p53. (A) Percentage survival from day 1 of organoids from the crypts of WT and Atg7−/− mice in the presence or absence of pifithrin in the culture medium (n = 3 mice of each genotype). Significant differences are shown for day 3. (B) Western blotting for ATG7, p53, and LC3 on whole-intestinal tissue lysates from WT, p53−/−, Atg7−/−, and Atg7−/−p53−/− mice. γ-Tubulin was used as a loading control. Results are shown for three individual mice of each genotype. (C) Representative p53, CC3, and TUNEL staining in the crypts of WT, p53−/−, Atg7−/−, and Atg7−/−p53−/− mice. Quantification of the mean number of TUNEL-positive cells per crypt over 50 consecutive whole crypts in seven WT, three p53, five Atg7−/−, and eight Atg7−/−p53−/− mice. The data shown are means ± SD. (Scale bars: 25 µm.) (D) Percentage survival from day 1 of organoids from the crypts of Atg7−/− and Atg7−/−p53−/− mice (n = 3 mice of each genotype). Significant differences are shown with asterisks. NS, not statistically significant. *P < 0.05; ***P < 0.005.