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. 2020 May 1;117(20):10673–10680. doi: 10.1073/pnas.1915078117

Fig. 2.

Fig. 2.

Symmetric reconstruction showing partially ordered RNA. (A and B) Interior radially colored view of the symmetric reconstruction showing the back half of the CP shell and either the entire (A) or the back half of (B) the RNA genome filtered to 10 Å and colored orange. The capsid symmetry axes are shown on the RNA genome for guidance. Notice that, in A and B, the RNA sits near the two- and threefold axes but not near the fivefold. No RNA density is resolved at the center of the particle. (C and D) Equatorial slab of the symmetric reconstruction without (C) and with (D) low-pass filtering to 10 Å. The two-, three-, and fivefold symmetry axes have been indicated, and it is clear that the RNA is situated preferentially near the two- and threefold axes and away from the fivefold axes. In fact, in the 10-Å structure, we see that the only resolved RNA–CP contact occurs between the RNA at the threefold axes and the hexameric N termini at these positions.