Fig. 8.

A transient breach in the intestinal barrier leads to RIP2-dependent increases in proportions of both CD103-expressing cDC1s and FoxP3-expressing Tregs. (A and B) Flow cytometry analysis of colonic LP cells isolated from Rip2^+/−mice and their Rip2^−/− littermates assessing the proportion of total DCs (A), CD11b⁺ or CD103⁺CD11b⁻ DCs (B), or Foxp3⁺ CD4 T cells (D) 3 d after administration of 100 μL of 50% ethanol via an intrarectal catheter. Individual mice from two (A and B) or three (D) pooled litters are shown. (C) The ratio of CD103⁺CD11b⁻ to CD11b⁺ DCs 3 d following administration of 100 μL of 50% ethanol via an intrarectal catheter. Horizontal bars denote the mean, and error bars denote SEM. Asterisks and brackets denote significantly different groups as calculated by two-way ANOVA followed by Tukey’s multiple comparisons test. *P ≤ 0.05, ***P ≤ 0.001. &P < 0.05, significantly different by t test alone. NS, nonstatistically significant differences between groups.