Fig. 6.
The utility of 4HPAAS in metabolic engineering of a shortened BIA pathway for (S)-norcoclaurine production in yeast. (A) Canonically proposed (S)-norcoclaurine biosynthetic pathway (black arrows) rerouted by the use of a 4HPAAS (red arrows). (B) Engineering of (S)-norcoclaurine production in yeast using two AAAD proteins with the 4HPAAS activity. All multigene vectors used to transform yeast contain BvTyH, PpDDC, and PsNCS in addition to either PsTyDC, PsTyDCY350F, or Rr4HPAAS. Cultures were grown and measured in triplicate. The error bars in the bar graph indicate SEM whereas the squares and triangles represent the individual data points; n.d., not detected.