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. 2020 Apr 30;117(20):10888–10896. doi: 10.1073/pnas.2000266117

Fig. 3.

Fig. 3.

The PER2-S478A mutation alters the expression profiles of clock genes and proteins in the liver. (A and B) Temporal expression of clock proteins in mouse liver. Liver cytoplasmic lysates (A) and nuclear extracts (B) were prepared at 4-h intervals and analyzed by SDS/PAGE and immunoblotting with indicated antibodies. Data are represented as dots for individual experiments and as lines for means. (C) CRY1 recruitment on the CLOCK complex in the liver nucleus. Liver nuclear extracts were subjected to immunoprecipitation with anti-CLOCK antibody, and the immunoprecipitates were analyzed by immunoblotting with the indicated antibodies. Data are represented as dots for individual experiments and as lines for means. (D) Temporal expression of clock genes. Livers were harvested at 4-h intervals followed by real-time RT-qPCR for the indicated mRNAs. Mean values ± SEM obtained from three animals of each genotype are given. P values: two-way ANOVA between WT and PER2-S478A (SA).