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. 2020 May 5;117(20):11126–11135. doi: 10.1073/pnas.1920935117

Fig. 4.

Fig. 4.

CMH treatment of preexisting EAE promotes apoptosis of infiltrated leukocytes. (A, C, and D). Frozen sections of lumbar spinal cord taken from EAE–normoxia or EAE–CMH mice at the peak (14 and 16 d) and remission (21 d) phases of disease were stained for CD45 (AlexaFluor-488) and cleaved caspase-3 (Cy-3) in A, DAPI and cleaved caspase-3 (Cy-3) in C, or DAPI and TUNEL (AlexaFluor-488) in D. (Scale bar, 50 μm [A and D] and 500 μm [C].) (B and E) Quantification of cleaved caspase-3+ (B) and TUNEL+ (E) percent area. Results are expressed as the mean ± SEM (n = 6 mice per group). Note that during the peak phase of disease (days 14–16), CMH strongly enhanced the death of infiltrated leukocytes. *P < 0.05, **P < 0.01.