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. 2020 May 5;117(20):11126–11135. doi: 10.1073/pnas.1920935117

Fig. 5.

Fig. 5.

Monocytes are the predominant type of leukocyte within EAE lesions. (A, B, and D). Frozen sections of lumbar spinal cord taken from disease-free, EAE–normoxia or EAE–CMH mice at the peak (14 and 16 d) and remission (21 d) phases of disease were stained for DAPI, CD45 (AlexaFluor-488), Mac-1 (Cy-3), or CD4 (Cy-3) in A, Mac-1 (Cy-3) in B, or CD4 (Cy-3) in D. (Scale bar, 50 μm [A] or 500 μm [B and D].) (C and E) Quantification of the total Mac-1+ (C) or CD4+ (E) area. Results are expressed as the mean ± SEM percent area (n = 6 mice per group). Note that during the peak phase of disease (days 14–16), monocytes are the most abundant type of leukocyte in inflammatory lesions and that CMH strongly enhanced monocyte death during the remission phase. *P < 0.05, **P < 0.01.