Figure 3. Binding of Cyt1Aa helices α−1 and α−2 mutants.
Biotin-labeled activated Cyt1Aa proteins were incubated with (lanes labeled as B: 1, 3, 5 and 7) or without (lanes labeled as P: 2, 4, 6, 8) Ae. aegypti BBMV, centrifuged and the pellets were loaded on SDS-PAGE, electro-transferred to PVDF membranes and biotinylated toxins were detected with streptavidin coupled to peroxidase as indicated in Materials and methods. Lanes correspond to, Cyt1Aa (lanes 1 and 2), Cyt1AaA65C (lanes 3 and 4), Cyt1AaL85C (lanes 5 and 6) and Cyt1AaN89C (lanes 7 and 8). Molecular weight markers are indicated in kDa in the left of the figure.