Table 2.
: Species-specific primers used for qPCR analysis of CDP and 6-4 photolyase, glucocorticoid receptor protein and Beta-actin (housekeeping) genes in Limnodynastes peronii larvae
| Gene Id | Accession number (reference sequence) | Limnodynastes peronii primers | Amplicon size (bp) |
|---|---|---|---|
| CPD photolyase (Class II CDP phr) | NP_001089127 CPD photolyase-like L homolog [Xenopus laevis] | F: CCT TGG GCG TAG TGT ATT G R: AGC CAA AGT GAC GGA TTG |
159 |
| 6-4 photolyase (6-4 phr) | NP_001081421–6-4 photolyase [Xenopus laevis] | F: CAA ACT GTC CTG GCA TCT ATC R: TGT CCC AGC TCC TCT AAT G |
140 |
| Glucocorticoid receptor protein (nr3c1) | ENA_CAA51010.1 glucocorticoid receptor protein [Xenopus laevis] | F: CCC TCT TCT CAA ATG GCT ATG R: GTT GTG CTG ACC TTC TAC TG |
164 |
| Beta-actin (β-actin) | Xenopus laevis actin, beta L homeolog (actb.L), mRNA | F: TGC GTG ACA TCA AGG AGA AG R: CAA GGA ATG AAG GCT GGA AG |
177 |