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. 2020 May 19;12:3661–3672. doi: 10.2147/CMAR.S243300

Figure 5.

Figure 5

HK2 was a direct target of miR-613. (A) Schematic of the miR-613-binding sequence in HK2 3ʹ-UTR and the mutant in the target sequence. (B) Relative luciferase activity in MCF-7 and BT549 cells transfected with HK2-3ʹ-UTR-WT or HK2-3ʹ-UTR-MUT and miR-NC mimic or miR-613 mimic. The expression of HK2 in 45 pairs of BC tissues and matched healthy breast tissues (C), clinical stage (I, II, III and IV) BC tissue samples (D), 3 pairs of BC tissues and matched healthy breast tissues (E), MCF-10A, MCF-7, and BT549 cells (F). (G) MiR-613 level by qRT-PCR in MCF-7 and BT549 cells transfected with miR-NC mimic or miR-613 mimic. (H) HK2 protein level by Western blot in MCF-7 and BT549 cells transfected with anti-NC, anti-miR-613, miR-NC mimic, or miR-613 mimic. (I) The correlations between circRAD18, miR-613 and HK2 mRNA expression were determined in BC tissues using the Spearman test. *P < 0.05.