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. 2020 May 19;8(1):e001009. doi: 10.1136/bmjdrc-2019-001009

Figure 3.

Figure 3

Results of promoted proliferation. (A) IF of pan-keratin in dermis (green object indicates pan-keratin positive, scale bar: 25 µm). (B) Statistical analysis of pan-keratin expression by IF in dermis (n=3). (C) Western blot of pan-keratin in wound section on days 7 and 31. (D) Statistical analysis of pan-keratin expression measured with Western blot (n=3). (E) Immunostaining of TGF-β in dermis on days 14, 25 and 31 (brown object indicates pan-keratin positive, scale bar: 25 µm). (F) Statistical analysis of TGF-β expression measured with IHC in dermis (n=3). (G) Western blot of TGF-β in wound section on days 14, 25 and 31. (H) Statistical analysis of TGF-β expression measured with Western blot (n=3). Compared with the control group, *p<0.05, **p<0.01. DAPI, 4',6-diamidino-2-phenylindole; FGF-21, fibroblast growth factor-21; F21–K2, FGF-21 and KGF-2; IF, immunofluorescence; KGF-2, keratinocyte growth factor-2; TGF-β, transforming growth factor-β.