Figure 4.

FAM110B inhibited the activation of Wnt/β-catenin signaling pathway. In A549 and H1299 cells, after transfected with FAM110B siRNA, cells were treated with 30nM XAV-939 or treated with DMSO as control for 24 hours at 37°C. Western blotting assay was performed using the indicated antibody and was normalized based on the protein level of GAPDH as a control (A, B). The corresponding changes of proliferation and invasion were determined by colony formation Assay) and matrigel invasion assay (C, D, 200×, scale bar = 50μm), respectively. *P < 0.05,**P < 0.01 (110B, FAM110B).