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. Author manuscript; available in PMC: 2021 May 21.
Published in final edited form as: Cell Chem Biol. 2020 Feb 13;27(5):511–524.e4. doi: 10.1016/j.chembiol.2020.01.011

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
Collagenase Worthington Biochemical Corporation Cat#LS004183
Ruthenium Red Millipore-Sigma Cat#R2751
Ru360 Millipore-Sigma, Calbiochem Cat#557440
ML335 Lolicato et al., 2017 N/A
Yeast Nitrogen Base (YNB) Invitrogen Cat#Q30007
Anti-GFP Nanobody Lolicato et al., 2017 N/A
3C Protease Shaya et al., 2011 N/A
Octyl glucose neopentyl glycol (OGNG) Anatrace Cat#NG31125GM
n-heptyl-β-D-thioglucopyranoside (HTG) Anatrace Cat#H30125GM
Cholesterol hemisuccinate (CHS) Anatrace Cat#CH21025GM
Phenylmethylsulfonyl fluoride (PMSF) Millipore-Sigma Cat#P7626
DNase I Roche Cat#1010415900
mMessage Machine T7 transcription kit ThemoFisher Cat# AM1344
RNEasy kit Qiagen Cat# 74104
Deposited Data
K2P2.1 (TREK-1) I110D (apo) structure This paper PDB: 6V36
K2P2.1 (TREK-1) I110D bound to RuR This paper PDB: 6V3I
K2P2.1 (TREK-1) I110D co-bound to RuR and ML335 This paper PDB: 6V37
K2P2.1 (TREK-1) I110D bound to Ru360 This paper PDB: 6V3C
K2P4.1 (TRAAK) G124I Lolicato et al., 2014 PDB: 4RUE
K2P2.1 (TREK-1) (apo) Lolicato et al., 2017 PDB: 6CQ6
K2P2.1 (TREK-1) bound to ML335 Lolicato et al., 2017 PDB: 6CQ8
K2P10.1 (TREK-2) (apo) Dong et al., 2014 PDB: 4BW5
Experimental Models: Organisms/Strains
Xenopus laevis (frogs for oocyte removal) Nasco Female, 9+CM
Pichia pastoris (SMD1163H) Lolicato et al., 2017 N/A
Recombinant DNA
Murine K2P2.1 (TREK-1) (pGEM) Bagriantsev et al., 2013 N/A
K2P2.1 (TREK-1) I110D (pGEM) This paper N/A
K2P2.1 (TREK-1) I110E (pGEM) This paper N/A
K2P2.1 (TREK-1) I110K (pGEM) This paper N/A
K2P2.1 (TREK-1) I110D/G137I (pGEM) This paper N/A
K2P2.1 (TREK-1) N147D (pGEM) This paper N/A
K2P2.1 (TREK-1) N147E (pGEM) This paper N/A
K2P2.1 (TREK-1) I110D/N147D (pGEM) This paper N/A
K2P2.1 (TREK-1) I110D/N147E (pGEM) This paper N/A
Murine K2P10.1 (TREK-2) (pGEM) Bagriantsev et al., 2013 N/A
Murine K2P9.1 (TASK-3) (pGEM) Bagriantsev et al., 2013 N/A
K2P2.1 (TREK-1)CRYST (pPicZ) Lolicato et al., 2017 N/A
K2P2.1 (TREK-1)CRYST I110D (pPicZ) This paper N/A
Software and Algorithms
pClamp, Version 8 Molecular Devices N/A
Clampfit 8.1 Molecular Devices N/A
Graphpad Prism, Version 5 Graphpad Software https://www.graphpad.com/scientific-software/prism/
XDS Kabsch, 2010 http://xds.mpimf-heidelberg.mpg.de/
Aimless Evans and Murshudov, 2013 http://www.ccp4.ac.uk/
PHENIX Adams et al., 2010 https://www.phenix-online.org/
COOT Emsley and Cowtan, 2004 http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
REFMAC Murshudov et al., 1997 http://www.ccp4.ac.uk/
Other
CNBr-activated sepharose resin GE Healthcare Cat#17-0430-01
Superdex 200 column, 10/300 GE Healthcare Cat#17-5175-01
Econo-Pac gravity column, 1.5 × 12 cm Bio-Rad Cat#7321010
GeneClamp 500B MDS Analytical Technologies N/A
Synchrotron radiation at APS GM/CAT Beamline 23-IDB/D APS, Chicago N/A