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. Author manuscript; available in PMC: 2021 May 21.
Published in final edited form as: Cell Chem Biol. 2020 Apr 23;27(5):622–634.e6. doi: 10.1016/j.chembiol.2020.03.006

Figure 4. In Vivo Verification of Select Stabilizing Molecules in the Eye and Liver.

Figure 4.

Wild-type non-pigmented BALB/c mice were intravitreally (A) or intravenously (C) injected with adeno-associated virus encoding for ecDHFR.firefly luciferase (ecDHFR.FLuc), assessed for baseline ocular/hepatic bioluminescence, and induced with 1 mg (50 mg/kg) of the indicated compound (via oral gavage). Bioluminescence was measured 6 h post gavage. Representative images of pralatrexate-treated mice are provided as examples (A and C). n = 4–7 mice per treatment. Mean fold change ± SEM is shown (B and D). *p < 0.05, **p < 0.01, ***p < 0.001, one-tailed t test versus control untreated mice. Note: a black tarp was used in (A) to block apparent background luminescence that can originate from the ear tag of the mice.