Figure 4.

Reduced fat mass in male obese mice during MR and apoptosis. Protein expression for Caspase 3 (CASP3) and Apoptosis-Inducible Factor (total protein: AIF 67 kDa and cleaved protein AIF 57 kDa) normalized to α-tubulin and their respective immunoblots from iWAT (A and B) and pgWAT (C and D) of WT DIO (A and C) and AF-dKO (B and D) mice fed either CF (white bars) or MR (black bars). Statistical analysis was conducted using Student’s t-test for each protein between CF and MR in each cohort, as described in Methods (n = 7 – 8 / group, *P < 0.05).