Figure 5.

MR induces an adipose tissue-depot specific autophagic response in male obese mice. Protein expression for ATG5, ATG7, Beclin1, LC3B, LAMP1 and LAMP1 normalized to α-tubulin and their respective immunoblots from iWAT (A and B) and pgWAT (D and E) of WT DIO (A and D) and AF-dKO (B and E) mice fed either CF (white bars) or MR (black bars). The ratios of LC3BII/I from iWAT of WT DIO (C) and pgWAT of AF-dKO (F). Statistical analysis was conducted using Student’s t-test for each protein between CF and MR in each cohort, as described in Methods (n = 7 – 8 / group, *P < 0.05).