Figure 6.

Weight loss in male obese mice improves glucose tolerance during MR. Glucose tolerance tests were conducted as described in Methods in (A) WT, (B) Adpn-KO (C) FGF21-KO, and (D) AF-dKO DIO mice that were fed either a CF or MR diet for 4 weeks. (E) Pyruvate and (F) insulin tolerance tests in AF-dKO DIO mice were conducted at 5 and 7 weeks, respectively, after diet initiation. Statistical analysis was conducted using 2-way ANOVA for time-course experiments or Student’s t-test for area under the curve (AUC), as described in Methods (n = 4 – 5 / group, **P < 0.001, *P < 0.05).