Table 2.

Blood biomarkers of male WT DIO mice and mice deficient in adiponectin (Adipoq-KO), FGF21 (Fgf21-KO), or both (AF-dKO) fed a high fat (DIO) CF or MR diet for 8 weeks. Comparisons between CF and MR in each cohort were conducted using Student’s unpaired t-test (n = 5 – 8 / group, *P < 0.05, **P < 0.01, ***P < 0.001). BHB, β-hydroxybutyrate; IGF-1, insulin-like growth factor-1. nd, not detected.

	WT DIO		Adipoq-KO DIO		Fgf21-KO DIO		AF-dKO DIO
	CF	MR	CF	MR	CF	MR	CF	MR
Triglyceride (mg/dl)	63 ± 5	53±8*	59 ±6	34 ± 5***	72 ± 7	65±9	43 ± 5	32 ± 4***
Cholesterol (mg/dl)	128 ± 14	84 ± 4***	162 ± 21	111 ± 7***	138 ± 28	98 ± 13*	144 ± 14	105 ± 17***
BHB (mM)	0.1 ± 0.03	0.16 ± 0.04**	0.18 ± 0.04	0.21 ± 0.05	0.1 ± 0.01	0.08 ± 0.02	0.19 ± 0.02	0.29 ± 0.06*
Glucose (mg/dl)	176 ± 25	96 ± 21***	164 ± 28	84 ± 5***	152 ± 22	77 ± 12***	145±7	64 ± 9***
Insulin (ng/ml)	4.2 ± 1.2	0.5 ± 0.4***	2.0 ± 1.6	0.2 ± 0.2*	2.5 ± 1.3	0.8 ± 0.5*	2.0 ± 1.2	0.4 ± 0.2
Leptin (ng/ml)	93±16	2.4 ± 0.8***	22 ± 13	1.4 ± 0.9*	13 ± 9.1	1.2 ± 0.7*	39 ± 29	1.4 ± 0.6**
IGF-1 (ng/ml)	475 ± 59	194 ± 35***	406 ± 93	158 ± 13***	431 ± 81	190 ± 25***	428 ± 39	194 ± 23***
Adiponectin (µg/ml)	10 ± 1.0	14 ± 1.8**	nd	nd	10 ± 1.9	15 ± 2.9**	nd	nd
FGF21 (ng/ml)	0.15 ± 0.1	3.4 ± 1.5**	0.20 ± 0.13	6.9 ± 4.12**	nd	nd	nd	nd

^aValues are mean ± SD.