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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Obesity (Silver Spring). 2020 Apr 13;28(6):1086–1097. doi: 10.1002/oby.22788

Figure 6 -. eWAT of formerly obese mice maintains elevated numbers of preadipocytes.

Figure 6 -

CD45−CD31−Sca-1+PDGFRα+ preadipocytes in normal diet (ND), weight loss (WL) and HFD diet (HFD) fed mice were quantified by flow cytometry in (A) eWAT (n = 4 for all conditions) and (B) iWAT (n = 4 for ND and n = 3 for WL and HFD). The quantity of Collagen 1 (Col1+) preadipocytes was determined by intracellular flow cytometry in (C) eWAT (n = 4 for all conditions) and (D) iWAT (n = 4 for ND and n = 3 for WL and HFD). * p < 0.05, *** p < 0.001, significance was only compared for ND versus WL or WL versus HFD (20wk).