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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Obesity (Silver Spring). 2020 Apr 13;28(6):1086–1097. doi: 10.1002/oby.22788

Figure 7 -. Conditioned media from eWAT explants of formerly obese mice inhibits adipocyte differentiation and reduces lipogenesis.

Figure 7 -

Gene expression for (A) adipocyte maturation genes and (B) collagen genes after differentiation induction in the presence of eWAT explant conditioned media from the respective diet conditions (n = 4). Oil red O staining of lipid droplets within differentiated adipocytes shown as (C) representative micrographs and (D) relative total extracted oil red O content from wells (n = 3). (E) eWAT explant multiplex cytokine array (n = 1 for ND and n = 2 for WL and HFD). (F) Extracted relative oil red O content from differentiated 3T3-L1 cells after treatment with (left) 10ng/mL of individual chemokines identified from multiplex cytokine array or (right) simultaneous treatment with all identified chemokines at 10ng/mL each (n ≥ 3). (G) Extracted relative oil red O absorbance after treatment with CM from ST-HFD, RC-HFD or LT-HFD with or without the addition of 50ng/mL neutralizing anti-TNFα antibody (αTNF) during differentiation (n ≥ 4). Undiff. = Undifferentiated Control, Diff. = Differentiated Control, All Chemok. = All identified chemokines. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p <0.0001, significance was only compared for ST-HFD versus RC-HFD, LT-HFD versus RC-HFD or ND versus WL unless otherwise noted.