Skip to main content
. Author manuscript; available in PMC: 2021 May 21.
Published in final edited form as: Mol Cell. 2020 Apr 20;78(4):653–669.e8. doi: 10.1016/j.molcel.2020.03.025
TADA1 KO and Rescue
sgRNA 5’ – GCCAAGAAGAACTTAAGCG – 3’
Genomic DNA 5’ – GCC AAG AAG AAC TTA AGC GAG GC– 3’
Rescue cDNA 5’ – GCC AAG AAG AAC TTA AGC GAA GC – 3’
Rescue cDNA sequence surrounding the PAM site mutation (in red, sgRNA sequence in yellow) ATGGCGACCTTTGTGAGCGAGCTGGAGGCGGCCAAGAAG
AACTTAAGCGAAGCCCTGGGGGACAACGTGAAACAATAC
TGGGCTAACCTAAAGCTGTGGTTCAAGCAGAAGATCAGC
AAAGAGGAGTTTGACCTTGAAGCTCATAGACTTCTCA