Table 2.

Software packages and libraries used within VADR

software and website	purpose in VADR
Bio-Easel v0.09 github.com/nawrockie/Bio-Easel	sequence alignment handling and sequence utility functions
Sequip v0.03 github.com/nawrockie/sequip	option handling, output file handling and other utilities
Infernal v1.1.3 github.com/EddyRivasLab/infernal	build and use profile HMMs and CMs to classify, validate, align and annotate sequences
BLAST+ v2.9.0 ftp.ncbi.nlm.nih.gov/blast/executables/blast+/2.9.0	build BLAST databases and validate CDS/protein predictions