Figure 8.

Effect of MAPK inhibitors on yolkin-mediated iNOS expression (a) and activation (b). BMDM cells were preincubated with MAPK inhibitors: U0126 for ERK and SP600125 for JNK for 2 h, followed by treatment with yolkin. Cells were lysed and expression of iNOS was determined by Western blot using monoclonal anti-iNOS antibodies (a). Supernatants were used to determine NO concentration by the Griess reaction (b). Results represent three independent experiments and present mean ± SD (n = 3). ^∗p ≤ 0.05 compared with the control, and ^∗∗p ≤ 0.05 and ^∗∗∗p ≤ 0.05 compared with yolkin.