Table-1.
Primer sequences, annealing temperatures, and PCR product sizes of the targeted genes conducted under this study.
| Gene | (Primers 5’®3’) | Annealing temperature | Restriction enzyme | Product size |
|---|---|---|---|---|
| BMPR-IB | F:GTCGCTATGGGGAAGTTTGGATG | 62°C | AvaII | 140 bp |
| FecB | R:CAAGATGTTTTCATGCCTCATCAACACGGTC | |||
| BMP15 | F:GCCTTCCTGTGTCCCTTATAAGTATGTTCCCCTTA | 62°C | DdeI | 153 bp |
| FecXB | R:TTCTTGGGAAACCTGAGCTAGC | |||
| BMP15 | F:CACTGTCTTCTTGTTACTGTATTTCAATGAGAC | 66°C | Hinf1 | 141 bp |
| FecXG | F:GATGCAATACTGCCTGCTTG | |||
| BMP15 | F:TATTTCAATGACACTCAGAG | 59°C | SpeI | 240 bp |
| FecXH | R:GAGCAATGATCCAGTGATCCCA | |||
| BMP15 | F:GAAAGTAACCAGTGTTCCCTCCACCCTTTTCT | 63°C | XbaI | 150 bp |
| FecXI | R:CATGATTGGGAGAATTGAGACC | |||
| GDF9-G1 | F- GAAGACTGGTATGGGGAAATG | 58°C | HhaI | 462 bp |
| R- CCAATCTGCTCCTACACACCT | ||||
| GDF9-G8 | F-CTTTAGTCAGCTGAAGTGGGACAAC | 62°C | DdeI | 139 bp |
| R-ATGGATGATGTTCTGCACCATGGTG | ||||
| TGAACCTGA | ||||
| PRL | F-ACCTCTCCTCGGAAATGTTCA | 56°C | HaeIII | 1211 bp |
| R-GGGACACTGAAGGACCAGAA |