Table-3.

Diagnosis methods based on modern molecular biology of opportunistic parasitic infection.

Parasite species	Molecular biology based methods of diagnosis	References
Cryptosporidium parvum	Monoplex PCR	[39]
	Multiplex PCR	[54]
	Lateral Flow Immunoassay, Immunochromatographic assay, Direct Fluorescent Antibody Test, TaqMan PCR	[22]
Cyclospora cayetanensis	Autofluorescent under UV EpiFluorescent, PCR	[22]
Isospora belli	PCR, Real time PCR	[22]
Blastocyst hominis	Phase contrast electron microscopy, serodiagnosis, PCR	[57,59]
Sarcocystis	Transmission Electron Microscopy	[55]
	PCR-RFLP	[56]
Microsporidia	Immunofluorescent Test, IFAT	[22]
	Monoplex PCR	[39]
	Immnuoblot, ELISA, IFAT	[23]
Cryptococcus neoformans	Multiplex PCR	[58]

PCR=Polymerase chain reaction, RFLP=Restriction fragment length polymorphism, IFAT=Immunofluorescence antibody test