Fig. 4.

DHT regulated mTOR-TFEB-NF-κB signaling pathway to ameliorate inflammation in DOX-stimulated cardiomyocytes. a Western blotting showed that DHT or rapamycin downregulated the expressions of phosphorylated mTOR and S6K in mice. N = 5 per group. All data were presented as means ± SD in triplicate. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 is significantly different as indicated, for values in the DOX group. b Western blotting showed that with MHY1485 co-treatment, the effect of DHT on inflammation-related proteins including TNF-α and COX2 was abrogated in cardiomyocytes. N = 5 per group. All data were presented as means ± SD in triplicate. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 is significantly different as indicated, for values in the DOX group. ^##p < 0.01, ^###p < 0.001 is significantly different as indicated, for values in the DOX + DHT group. c Cardiomyocytes were transfected with GFP-TFEB and results showed that co-treatment with MHY1485 abolished the effects DHT on the nuclear localization of TFEB, scale bar = 50 μm. N = 20 per group