Fig. 5.

DHT inhibited NF-κB transcriptional activity via TFEB-IKK signaling pathway. a Cardiomyocytes were transfected with Lentiviral vector (Lv) carrying GFP-TFEB to monitor the location of TFEB. Lv-GFP transfected cells were set as a negative control. Representative GFP fluorescence and immunofluorescence staining of p-NF-κB were shown under different treatment, scale bar = 100 μm. N = 20 per group. b, c, d Western blotting showed that DOX reduced the nuclear expression of TFEB and increased phosphorylated levels of IKKα/β and NF-κB, while DHT up-regulated the nuclear expression of TFEB. In particular, TFEB overexpression or DHT treatment down-regulated phosphorylated levels of IKKα/β and NF-κB. N = 3 per group. e Representative GFP-TFEB fluorescence and immunofluorescence staining of p-NF-κB under different treatment, scale bar = 50 μm. N = 20 per group. All data were presented as means ± SD in triplicate. *^*p < 0.05, ^**p < 0.01, ^***p < 0.001