Fig. 3.

MSI2a negatively regulates TNBC cell growth and migration in vitro.a Western blot and qRT-PCR assays. In MDA-MB-231 and MDA-MB-468 cells, MSI2a transfection significantly increased MSI2a mRNA and protein expression but did not affect MSI2b expression. MSI2b transfection significantly increased MSI2b mRNA and protein expression but did not affect MSI2a expression. b CCK-8 assay for cell viability. Cell proliferation was assessed after MSI2a and -b overexpression in MDA-MB-231 and MDA-MB-468 cells c Wound-healing assay. Images of the wound-healing assay data showing the wound-healing ability in MSI2a- and -b-overexpressing MDA-MB-231 and MDA-MB-468 cells. Scale bar, 200 μm. d Transwell migration assay. Images showing the migration capacity in MSI2a- and -b-overexpressing MDA-MB-231 and MDA-MB-468 cells. Scale bar, 100 μm. e qRT-PCR assays. The efficiency of MSI2a knockdown in BT20 and MDA-MB-231 cells. f CCK-8 assay for cell viability. Cell proliferation was assessed after MSI2a knockdown in BT20 and MDA-MB-231 cells. g Transwell migration assay and wound-healing assay. Images showing the migration capacity and wound-healing ability of MSI2a-knockdown BT20 and MDA-MB-231 cells