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. 2020 May 24;547:35–46. doi: 10.1016/j.virol.2020.05.005

Fig. 1.

Fig. 1

Characterization of SPONV in non-pregnant Ifnar1−/−mice. (a) Serum was collected from mice during three independent replicates at 2, 4, and/or 6 days post inoculation and titered via plaque assay. Assay limit of detection was 100 PFU. Viremia peaked at 4 dpi for all virus groups, with ZIKV-DAK replicating to significantly higher titers at 4 dpi than SPONV (one-way ANOVA). ****p < 0.0001; ***p < 0.0005; **p < 0.006 (b) Survival curves of six-to eleven-week old Ifnar1−/− mice s.c. inoculated with 103 PFU of SPONV, 102 PFU of SPONV, 102 PFU ZIKV-DAK, or a PBS control. SPONV 103: n = 14; SPONV 102: n = 13, ZIKV-DAK: n = 5; PBS: n = 6). All virus infections caused significant mortality by 14 dpi as compared to PBS controls (Fisher's exact test). ***p < 0.0005; **p < 0.005.