Fig. 3.

DENV outcomes in non-pregnant and pregnant Ifnar1^−/−mice. (a) Serum viral loads from non-pregnant (black symbols) and pregnant (orange) Ifnar1^−/− mice at 2 and 4 days post infection with 7.5 × 10⁴ PFU of DENV. Viral loads were measured by QRT-PCR. All mice had detectable serum viremia at both 2 and 4 dpi. (b) Rates of grossly abnormal (red) versus normal (black) fetuses at E14.5 after maternal infection at E7.5. Data presented are for individual fetuses from 6 litters from DENV infected dams. Resorption rates did not significantly differ between DENV and PBS controls. ns, not significant (Fisher's exact test). (c) Viral titer was measured by plaque assay for a subset of individual homogenized fetuses and placentas. There was no detectable infectious DENV virus present in any tested tissue.