Fig. 5.

Fetus histopathology analysis: Hematoxylin and eosin (H&E) staining of fetal brain and spinal cord. (a–d) Fetal brains at E14.5 from PBS- (a), SPONV- (b), ZIKV- (c), and DENV- (d) inoculated dams. Brains from PBS- and DENV-inoculated animals showed no signs of pathology. Significant histopathologic injury was observed in fetal brains from SPONV- and ZIKV-inoculated dams, as indicated by large necrotic regions (N). Scale bar (a–d), 500 μm. (e–h) Spinal cord at E14.5 from PBS (e), SPONV (f), ZIKV (g), and DENV (h) inoculated dams. Fetal spinal cords from ZIKV and SPONV infections exhibited histopathologic injury as indicated by necrosis (N). Scale bar (e–h), 100 μm.