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. Author manuscript; available in PMC: 2020 May 24.
Published in final edited form as: J Vis Exp. 2019 Nov 13;(153):10.3791/60365. doi: 10.3791/60365

Figure 2. Microfluidic system.

Figure 2.

The two-valve configuration allows control of flow direction towards the microinjectrode or towards the flushing line for troubleshooting. The circuit relies on two 3-port valves connected using capillary tubing and standard ferrules. Gastight syringes are used to carry and inject the infusion drug and the marker. A programmable syringe pump allows for automatic flushing of the system and loading of the drug. A manual microsyringe pump allows for controlled injection and visualization.